Application

Research CategoryApoptosis & Cancer

Western blot: 1:500-1:1000

Immunoprecipitation

Whole cell lysate from HeLa cells can be used as a positive control and a 45 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Research Sub CategoryApoptosis - Additional

This Anti-DFF45/35 Antibody, N-terminus is validated for use in IP, WB for the detection of DFF45/35.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: N-terminus

Polyclonal antibody raised against a peptide corresponding to amino acids 2-21 at N-terminus of human DFF (Liu et al. 1997).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Specificity

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (Liu et al. 1997). Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD (Enari et al. 1998; Sakahira et al. 1998). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (Enari et al. 1998; Sakahira et al. 1998). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which cause DNase activation and DNA degradation, is the hallmark of apoptotic cell death (Wylile et al. 1998).

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.